Coding
Part:BBa_K2570012:Design
Designed by: Luying He Group: iGEM18_FJNU-China (2018-09-30)
D-ldh
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 773
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The 52th tyrosine is mutated to leucine.
Mutation of restriction sites EcoRⅠand PstⅠ.
Source
D-lactate dehydrogenase Dldh from Lactobacillus bulgaricus ATCC 11842